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Determination of NADP+-ME (A) and NAD+-ME (B) activity. ME activity was assayed in cell extracts of M145, TR2951, TR5261, TRD strains and in their corresponding complemented strains grown on SMM-Glc for 30 h. Values represent the average of experiments performed in triplicate ± SD. N.D., not detected. Different letters denote statistical differences applying an ANOVA test using Sigma Stat 3.1 (P < 0.05).
