Fig 6.

Lipid analysis of ME mutants and M145 parent strain of S. coelicolor. Total lipids were extracted with chloroform and methanol, separated by TLC, and visualized by Cu²⁺-phosphoric staining. TAG content was determined by densitometry. Values represent TAG content relative to total lipids for the M145, ME mutant, and their complemented derivative strains at three time points (20, 24, and 30 h). Values represent the average of three independent experiments ± SD. Asterisks denote statistical differences applying Student's t test (P < 0.05).