Table 1.

M. agalactiae isolates from wild fauna included in the study, isolation details, and characterization by MF-dot, mobilome composition, and VNTR profile

Source and isolate no.	Description	Isolation					Antigenic signatured		Mobilome		VNTR profilee
		Date (mo/yr)	Sourcea	Other bacteria isolated from lungs	Host age (yr)b	Probable cause of deathc	M. mycoides subsp. capri PG3	M. agalactiae PG2	Phage	ICE	5	14	17	19	St
Capra ibex
13387		07/03	L	Mannheimia haemolytica, Arcanobacterium pyogenes	14	P, K	++	+/−	−	+	p0	p0	p0	p2	St02
13501		09/03	L	None	3	P	+/−	+/−	−	+	p0	p0	p0	p2	St02
14628		03/06	L	M.haemolytica, A. pyogenes	11	P	+++	+	+	+	p0	p0	p0	p2	St02
14797		05/07	L	M. haemolytica, A. pyogenes	5	P, K	++	+	+	+	p0	p0	p0	p2	St02
14934		01/08	L	Pasteurella multocida, Streptococcus spp.	NK	P, K	−	+++	+	+	p0	p0	p0	p2	St02
14944		02/08	L	M. haemolytica	NK	P, K	−	+	+	+	p0	p0	p0	p2	St02
14980		03/08	EC	P. multocida	1	P	+++	++	−	+	p0	p0	p0	p2	St02
14989		04/08	EC	P.multocida, A. pyogenes	NK	P	++	+	+	+	p0	p0	p0	p2	St02
15009		04/08	L	P. multocida	11	P	+	+++	−	+	p0	p0	p0	p2	St02
15027		05/08	EC	None	2	Capture only	−	−	+	+	p0	p0	p0	p2	St02
15044		05/08	Uterus	P. multocida, Streptococcus spp.	5	P	+/−	+	+	+	p0	p0	p0	p2	St02
15179		12/08	L	None	3	K	+++	++	−	+	p0	p0	p0	p2	St02
15196		01/09	L, N	P. multocida, A. pyogenes	6	P	+/−	++	+	+	p0	p0	p0	p2	St02
15201		02/09	N	P. multocida	14	P	++	+	−	+	p0	p0	p0	p2	St02
15261		04/09	L	A. pyogenes	11	Enterotoxemia	+++	+	−	+	p0	p0	p0	p2	St02
15310		06/09	L	Streptococcus spp.	7	P	−	++	−	+	p0	p0	p0	p2	St02
15406		04/10	L, N	P. multocida, A. pyogenes, Micrococcus spp.	NK	P	+++	+/−	−	+	p0	p0	p0	p2	St02
15409		04/10	EC	Staphylococcus spp., Corynebacterium spp.	NK	Capture only	+++	+/−	+	+	p0	p0	p0	p2	St02
Chamois
15341		12/09	N	M. haemolytica, P. multocida	Young	P, K	−	+++	−	+	p0	p0	p0	p2	St02
15379		03/10	L, N	P. multocida, M. haemolytica	Adult	P, K	−	+++	−	+	p0	p0	p0	p2	St02
Reference strains
PG2	Type strain (goat, 1952, Spain)						−	+++	−	−	p1	p1	p1	p1	St06
5632	Sequenced strain (goat joint, before 1991, Spain)						−	++	−	+	p1	p0	p5	p1	St13
4908	Reference for the historical CA in the Savoys (goat milk, before 1990)						−	++	−	−	p1	p1	p1	p0	St05
4206	Reference for the area of CA endemicity in the Pyrénées Atlantiques (ovine milk, 1981)						−	++	−	−	p1	p1	p3	p1	St10

^aL, lung; EC, ear canal; N, nares.

^bNK, not known.

^cP, pneumonia; K, keratoconjunctivitis.

^dAntigenic profiles were determined by MF-dot using antisera prepared against the type strain of M. mycoides subsp. capri (PG3) or of M. agalactiae (PG2). The intensities of the MF-dot reaction were noted as follows: −, negative; +/−, doubtful; +, weak; ++, average; +++, strong.

^eVNTR profiles and share types (St) are numbered as proposed by L.-X. Nouvel et al. (20).