Table 2.

Ten MLST loci used in this study and primers used to amplify and sequence them

Locusa	Geneb	Gene size (bp)	Gene product	Primer	Sequence (5′→3′)c	Tm (°C)d	Positionse	PCR product size (bp)
terS	orf1	525	Terminase small subunit	terS-F	TAAGATGTAYGAAGAACCGC	50.9	312–716	405
				terS-R	TTAAGGTCATKAGCGCTTG	51.4
terL	orf2	1,623	Terminase large subunit	terL-F	GATGATTTTAGGCGGACAAT	50.5	1149–1732	584
				terL-R	AGAACTTATTCTGTAACGCTG	50.1
por	orf4	1,137	Portal protein	por-F	ATTCAAACTAAGCTGGAACA	49.5	3185–3646	462
				por-R	TTCTTTCAAAGTTGCAAACTT	49.1
mcp	orf6	1,182	Major capsid protein	mcp-F	GCAAAACTTGCTGAAAATGG	51.0	4675–5259	585
				mcp-R	CTCATCTAACAAGGCTTTACG	51.0
mtp	orf11	906	Major tail protein	mtp-F	TARCTGGTTTAGTATCRGTTGG	51.9	6942–7296	355
				mtp-R	ACTGAWTCTGTTTCTGATTCTT	49.7
tmp	orf14	3,000	Tail measure protein	tmp-F	AAYTTACAAACGCAGTTGG	50.0	8767–9358	592
				tmp-R	CAAAYGCTTGGTTAGCTTTTA	50.5
bpl	orf16	1,128	Baseplate protein	bpl-F	GACACGGAAACAATARYAGAAC	51.7	13178–13602	425
				bpl-R	GGYTTGTCTCCACTTTCTAC	51.7
rbp	orf18	795	Receptor-binding protein	rbp-F	CCAGTCGGTKCTAATAATGA	50.5	13936–14788	853
				rbp-R	GGTTCATYGCTTCTCTATCTT	51.0
lys	orf20	762	Endolysin	lys-F	AACAAACGGAGGATAAAAAAG	49.0	15032–15511	480
				lys-R	AGTTCTGYRATAAARTATGAGC	49.4
orf33	orf33	216	Unknown	orf33-F	TCGCCAAGAATTTCATCAG	50.4	19647–20145	499
				orf33-R	GAATCAGGCGARTACGTAAA	51.1

^aLoci shown in boldface type compose the final MLST scheme.

^bGene name according to the phage p2 genome.

^cA, deoxyadenosine; C, deoxycytidine; G, deoxyguanosine; T, thymidine; K, T+G; R, A+G; W, A+T; Y, C+T.

^dT_m, melting temperature.

^eNumbers denote the positions of the first and last bases of the locus on the phage p2 genome.