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Comparison of Wolbachia density in G. fuscipes fuscipes (Gff) with that in a laboratory strain of G. morsitans morsitans (Gmm). Wolbachia densities from female Wolbachia-positive G. fuscipes fuscipes flies (GffWol+) (n = 12), Wolbachia-negative G. fuscipes fuscipes flies (GffWol−) (n = 4), and Wolbachia-positive G. morsitans morsitans flies (GmmWol+) (n = 4) were measured by qPCR and normalized by tsetse β-tubulin (n ≥ 4). *, P < 0.05; ***, P < 0.0001.
