Table 1.

Bacteriophage insertion site primers

Target	Primer	Sequence	Cycle conditions
argW left junction	int4045F	5′-ACCATCGAGTAGGCGGTATG-3′	96°C for 10 s, 60°C for 30 s, 72°C for 40 s
	int1810R	5′-ATTTCAGCAGGGCCAGAGTA-3′
argW right junction	yfdCF	5′-ACTGGAGCGATTTCATCTGG-3′	96°C for 10 s, 60°C for 30 s, 72°C for 40 s
	phi1810stx2F	5′-GGTTGAGCGGGATATGAAAA-3′
sbcB left junction	sbcBF	5′-ATTGTCGCGCTAAAGCTGAT-3′	96°C for 10 s, 60°C for 30 s, 72°C for 45 s
	stx2cphiB	5′-CAACGATGCTCGTTATGGTG-3′
sbcB right junction	stx2cphiA	5′-GGACAACAGCGCACAGTAAA-3′	96°C for 10 s, 60°C for 30 s, 72°C for 45 s
	sbcBR1	5′-CGGGCTTGCAGTAAAAGACT-3′
prfC	prfCF	5′-CGATGCCGCTTACTCAAGA-3′	96°C for 10 s, 60°C for 30 s, 72°C for 45 s
	prfCR	5′-GAACAGCAGCACCTTCTCG-3′
yecE	yecEF	5′-ATTGCCGAAGATGCCTGTAG-3′	96°C for 10 s, 60°C for 30 s, 72°C for 45 s
	yecER	5′-CATACAGCGCGCTTACCATA-3′