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Binding of AbrB to the promoter region of pmxA. The promoter region of pmxA (PpmxA; 250 bp) was labeled with biotin. The biotin-labeled PpmxA (10 ng) interacted with 500 nM AbrB in a binding buffer containing 50 μg of poly(dI-dC) per ml as the nonspecific competitor. Lanes: 1, biotin-labeled PpmxA only; 2, mixture of biotin-labeled PpmxA and AbrB; 3 and 4, mixture of biotin-labeled PmxA, AbrB, and non-biotin-labeled PpmxA (5- and 10-fold for each lane).
