. 2012 Jun;78(12):4271–4280. doi: 10.1128/AEM.00309-12

Table 4.

Effects of different doses of essential oils on biodiversity indices from DGGE fingerprints of the rumen bacterial and archaeal communities in vitro^a

Essential oil and dose (g/liter)	Bacteria			Archaea
Essential oil and dose (g/liter)	Shannon index	Evenness index	Dominance index	Shannon index	Evenness index	Dominance index
Control, 0	2.76	0.964	0.069	2.20	0.895	0.130
CLO
0.25	2.87	0.942	0.065	2.16	0.869	0.137
0.50	2.85	0.933	0.066	2.05	0.855	0.160
1.00	2.79	0.952	0.068	1.96	0.879	0.162
SEM	0.041	0.0051	0.0029	0.037	0.010	0.0076
Contrast	Q^*	Q^***	NS	L^***	Q^*	L^***
EUO
0.25	2.86	0.948	0.063	2.30	0.917	0.113
0.50	2.91	0.967	0.058	2.33	0.901	0.113
1.00	2.84	0.960	0.063	2.12	0.844	0.150
SEM	0.039	0.0048	0.0026	0.034	0.007	0.0053
Contrast	Q^**	C^*	Q^**	L,^* Q^***	L,^* Q^*	L,^* Q^*
GAO
0.25	3.01	0.957	0.054	1.92	0.921	0.162
0.50	2.87	0.943	0.063	1.92	0.872	0.167
1.00	2.72	0.920	0.074	1.92	0.836	0.172
SEM	0.039	0.0041	0.0026	0.029	0.013	0.0057
Contrast	L,^* Q,^* C^*	L^***	L,^ Q,^* C^**	L,^* Q,^* C^**	L,^*** C^*	L,^* Q^
ORO
0.25	2.73	0.928	0.073	2.04	0.850	0.158
0.50	2.75	0.918	0.073	1.79	0.863	0.188
1.00	2.58	0.932	0.085	1.14	0.708	0.398
SEM	0.035	0.0053	0.0025	0.036	0.021	0.013
Contrast	L^***	L,^* Q^*	L^***	L,^* Q^	L,^*** Q^*	L,^* Q^*
PEO
0.25	2.82	0.946	0.066	2.18	0.877	0.138
0.50	2.79	0.943	0.068	1.91	0.871	0.170
1.00	2.84	0.949	0.064	2.00	0.912	0.151
SEM	0.031	0.0038	0.0020	0.032	0.014	0.0067
Contrast	NS	L,^* Q^***	NS	L,^* Q,^* C^***	Q^*	L,^ Q,^ C^*

Data were analyzed using dose levels of 0, 0.25, 0.50, and 1.0 g/liter for each essential oil. CLO, clove oil; EUO, eucalyptus oil; GAO, garlic oil; ORO, origanum oil; PEO, peppermint oil; NS, not significant. Only significant (***, P ≤ 0.01; **, P ≤ 0.05; *, P ≤ 0.10) linear (L), quadratic (Q), and cubic (C) effects are shown.