Table 2.
Operational taxonomic units within five ANG 16S clone librariesa
| Phylotype | No. of OTUs |
||||
|---|---|---|---|---|---|
| ANG1 | ANG2 | ANG3 | ANG4 | ANG5 | |
| Alphaproteobacteria | |||||
| Rhodobacterales | |||||
| Phaeobacter | 20 | 49 | 78 | 40 | 26 |
| Ruegeria | 9 | 6 | 0 | 6 | 4 |
| Nautella | 2 | 4 | 0 | 5 | 1 |
| Labrenzia | 0 | 6 | 0 | 1 | 0 |
| Pseudoruegeria | 0 | 1 | 0 | 0 | 0 |
| Oceanicola | 0 | 1 | 0 | 0 | 0 |
| Marinovum | 0 | 0 | 2 | 0 | 0 |
| Salipiger | 0 | 0 | 4 | 0 | 0 |
| Rhizobiales | |||||
| Cohaesibacter | 0 | 1 | 0 | 2 | 3 |
| Mesorhizobium | 0 | 0 | 2 | 0 | 0 |
| Kordiimonadales | |||||
| Kordiimonas | 1 | 4 | 0 | 3 | 4 |
| Verrucomicrobia | 37 | 12 | 3 | 25 | 29 |
| Gammaproteobacteria | |||||
| Shewanella | 0 | 1 | 0 | 0 | 0 |
| Total no. of clones | 69 | 85 | 89 | 82 | 67 |
a
A total of 392 clones were binned into taxonomic groups by RFLP analysis. The full-length 16S rRNA gene of 71 clones from ANG1, ANG2, and ANG3 was analyzed using the Greengenes database (11) after chimeric sequence removal (Materials and Methods).