Fig 4.

Comparison of G1mgNtBG1 and Novozym 188. (A) For stability testing, G1mgNtBG1 (closed diamonds) and Novozym 188 (open circles) were incubated in 50 mM sodium acetate buffer (without substrate) at 60°C for the times indicated and their residual activities were measured. (B) Several concentrations of glucose were incubated with G1mgNtBG1 (closed diamonds) and Novozym 188 (open circles), and their activities were measured according to the standard assay method using pNPG as the substrate. Data are expressed as the mean ± the standard deviation of three independent experiments.