Fig 5.

Enhanced production of reducing sugars from Avicel by addition of β-glucosidases. Enzyme solution was incubated at 50°C for the times indicated. As a control, Celluclast 1.5 L (closed diamonds) was mixed with the substrate. Addition of G1mgNtBG1 (closed triangles) and Novozym 188 (open squares) to Celluclast 1.5 L enhanced the production of reducing sugars (*, P < 0.05; **, P < 0.01 [Student's t test]). Reducing sugar was measured with DNS reagent. Data are expressed as the mean ± the standard deviation of three independent experiments.