Table 1.
Relative activity of G1mgNtBG1 on various substrates
| Substrate | Linkage of glycosyl group | Mean relative activity (%) ± SDa |
|---|---|---|
| Aryl glycosides | ||
| pNPFuc | βFuc | 100.0 ± 0.44 |
| pNPG | βGlc | 37.5 ± 0.45 |
| pNPLac | βLac | 13.3 ± 0.31 |
| pNPGal | βGal | 9.1 ± 0.42 |
| Saccharides | ||
| Laminaribiose | (β-1,3) Glc | 100.0 ± 2.18 |
| Sophorose | (β-1,2) Glc | 92.2 ± 1.83 |
| Lactose | (β-1,4) Gal | 69.9 ± 2.95 |
| Salicin | βGlc | 11.7 ± 0.84 |
| Gentiobiose | (β-1,6) Glc | 10.3 ± 0.16 |
| Laminarin | (β-1,3; β-1,6) Glc | 6.5 ± 0.58 |
| CMC | (β-1,4) Glc | 0.0 ± 0.00 |
| Avicel | (β-1,4) Glc | 0.0 ± 0.00 |
| Cellobiose | (β-1,4) Glc | 98.2 ± 2.29 |
| Cellotriose | (β-1,4) Glc | 32.2 ± 4.00 |
| Cellotetraose | (β-1,4) Glc | 24.5 ± 2.34 |
| Cellopentaose | (β-1,4) Glc | 19.7 ± 3.55 |
| Cellohexaose | (β-1,4) Glc | 15.5 ± 1.35 |
a
The release of sugars was measured at A410 for aryl glycosides and at A505 for saccharides. The relative activity of the most preferentially hydrolyzed substrates was taken as 100%. Each value is the mean of triplicate experiments.