Fig 2.

Susceptibility of L. lactis strains to cell wall-active antimicrobials. (A) Susceptibility of different L. lactis cell envelope mutants to the bacteriocin Lcn972. Twofold dilutions of Lcn972 in 50 mM sodium phosphate buffer, pH 6.8, were spotted (5 μl) on L. lactis lawns. (B) Susceptibility of L. lactis MG1614 (WT) and the Lcn972-resistant strains L. lactis D1 and D1-20 to lysozyme (Lys), nisin (Nis), bacitracin (Bac), and penicillin G (PG). Tenfold dilutions of overnight cultures (5 μl) were spotted on GM17 plates containing the indicated concentrations of the antimicrobials.