Table 1.
Bacterial strains, bacteriophages, and plasmids used in this work
| Strain, phage, or plasmid | Descriptiona | Reference/source |
|---|---|---|
| L. lactis | ||
| MG1363 | Plasmid-free and prophage-cured derivative of L. lactis NCDO712 | 13 |
| MG1614 | Strr Rifr derivative of MG1363, Lcn972s | 13 |
| NZ9000 | MG1363, carrying pepN::nisRK | 21 |
| D1 | MG1614, Lcn972 high-resistant mutant, unstable | This work |
| D1-20 | MG1614, Lcn972 low-resistant mutant derived from D1, stable | This work |
| ΔdacA strain | NZ9000 lacking dacA gene | This work |
| ΔdacB strain | MG1363 lacking dacB gene | 9 |
| ΔdltD strain | MG1363 lacking dltD gene | 10 |
| MGRrF | MG1363 pRV300::llrF | 30 |
| E. coli | ||
| DH10B | Plasmid free, cloning host | Invitrogen |
| Phages | ||
| c2 | L. lactis lytic phage belonging to c2 family | E. Bidnenko |
| sk1 | L. lactis lytic phage belonging to 936 family | E. Bidnenko |
| Plasmids | ||
| pORI280 | EmrlacZ+, integrative plasmid | 22 |
| pBL16 | 600-bp dacA flanking regions cloned in pORI280 | This work |
a
Str, streptomycin; Rif, rifampin; Em, erythromycin.