Fig 3.
(A) Analysis of Porf1 promoter activities in the HD-73 wild-type strain (●), the sigE mutant (■), and the sigK mutant (△). The promoter-directed synthesis of β-galactosidase in the strains was determined at the indicated times after growing the cells in SSM at 30°C. Each value represents the mean for at least three independent replicates. (B) The promoter region of the operon. Transcriptional initiation (+1) is marked. The putative promoter region of the operon (−35 and −10) and the putative ribosome binding site of orf1 (RBS) are underlined and marked. “orf1” below the arrow indicates the 5′-end of orf1 and the deduced amino acid sequence. (C) Sequence similarity among the cry gene promoters and the consensus sequences obtained by aligning the σE-dependent promoters of B. thuringiensis. “*” indicates the transcriptional initiation sites. Consensus sequences of σE-dependent promoters are presented above and below the alignment, respectively. D is A, G, or T; H is A, C, or T; K is G or T; M is A or C; N is A, C, G, or T; V is A, G, or C.