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Effects of pH on β-glucanases expressed by Escherichia coli BL21(DE3) harboring pET21a vectors containing the bglA13 (a), bglA16 (b), bglA51 (c), bglM2 (d), bglS (e), and bglR (f) genes. The optimal reaction pH values of the β-glucanases were analyzed in different buffers with specific pH values (pH 3 to 10). Enzyme activities are compared to the largest activity value attained (i.e., 100%).
