Fig 7.

Role of EndA in biofilm formation under hydrodynamic conditions. (A) (Left) The formation of biofilms by Gfp-tagged wild-type and ΔendA and ΔexeM nuclease mutant strains under hydrodynamic conditions was monitored by CLSM after 48 h of incubation. (Center) eDNA was then visualized by DDAO staining. (Right) The corresponding overlays are displayed. (B) A wild-type biofilm formed after 24 h in flow chambers was treated with MBP-EndA (center) or DNase I (right) for 45 min. The lateral edge of each micrograph measures 250 μm.