Fig 5.

Viability quantification of S. tropica CNB-440 using the BacLight bacterial viability kit. (A) Fluorescence emissions in the viable (510- to 540-nm) and dead (620- to 650-nm) wavelengths for different ratios of live and dead cells along with the WT strain and the mscL⁺ transformant following 24 h of exposure to DI water. (B) The integrated 510- to 540-nm and 620- to 650-nm fluorescence ratio for the WT following a 24-hour exposure to DI water corresponds to ca. 20% viable bacteria, while that for the mscL⁺ exconjugant corresponds to greater than 80% viable bacteria. Averages ± standard deviations for three replicate experiments are plotted.