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Demonstration of the mycolactone plasmid. Pulsed-field gel electrophoresis (A) and Southern hybridization (B) analyses of MU Agy99 (lanes 1 and 2) and MU1615 (lanes 3 and 4), showing the presence of the linearized form of the plasmid in nondigested genomic DNA (lanes 1 and 3) and after digestion with XbaI (lanes 2 and 4), hybridized to a combination probe derived from mlsA, mlsB, mup038, and mup045. Lane M is the lambda low-range DNA size ladder (NEB).
