Fig. 2.

Circular representation of pMUM001. The scale is shown in kilobases by the outer black circle. Moving in from the outside, the next two circles show forward and reverse strand protein-coding DNA sequences, respectively, with colors representing the functional classification (red, replication; light blue, regulation; light green, hypothetical protein; dark green, cell wall and cell processes; orange, conserved hypothetical protein; cyan, insertion sequence elements; yellow, intermediate metabolism; gray, lipid metabolism). These circles are followed by the GC skew (G – C)/(G + C) and finally the G + C content by using a 1-kb window. The arrangement of the mycolactone biosynthetic cluster has been highlighted, and the locations of all XbaI sites are indicated.