Table 1.
Effects of denaturing reagents on activities of mPls and mPlsΔC740
| Reagent | Relative activity (%)a |
|||
|---|---|---|---|---|
| mPls |
mPlsΔC740 |
|||
| Suc-AAPK-pNA | Azocasein | Suc-AAPK-pNA | Azocasein | |
| None | 100 | 100 | 100 | 100 |
| SDS (2%) | 21 | 103 | 19 | 88 |
| Urea (6 M) | 13 | 219 | 11 | 184 |
| SDS (2%) + urea (6 M) | 6 | 152 | 2 | 138 |
a
The enzyme activity on suc-AAPK-pNA and azocasein was measured at 90°C and 95°C, respectively. The relative activity values were calculated based on the measured activity in the absence of denaturing regent (defined as 100%). The values are expressed as means of the results of three independent experiments, and standard deviations were less than 10% of the means.