Fig 4.

Neighbor-joining tree showing the phylogenetic analysis of the derived amino acid sequences encoded by pmoC. The amino acid sequences of PmoC used in the tree construction were deduced from the singleton copies and pmoCAB operons present in the genomes of Mc. capsulatus Bath, Mb. tundripaludum SV96, Ms. trichosporium OB3b, Methylocystis sp. strain Rockwell, Methylocystis sp. strain SC2, Methylocystis sp. strain M, and “Ca. Methylacidiphilum infernorum” V4. The accession numbers of the respective PmoC proteins are given in parentheses. Singleton pmoC copies of all the methanotroph strains are marked with “s” in their designations. Singletons from strain SC2 are labeled with “G” or “P” to distinguish between the genome- and plasmid-carried copies. AmoC sequences from Nitrosomonas europaea ATCC 19718 (NC_004757) were also included. Bootstrap values above 50 are shown. The scale bar represents 0.05 substitutions per amino acid position.