Table 3.

Parameters of SC and OPC methods used for sample quantification

Template	Primer pair	SC method					OPC method
		Slope	Intercept	R2	nc	Eds	CT	N0	Efi
G. sulfurreducensa	nifHF/nifHR	−4.249	40.86	0.993	8	1.72	22.2	22,160	1.69
N. communea	nifHF/nifHR	−3.552	36.79	0.991	8	1.91	24.8	2,331	1.77
B. japonicumb	nifHF/nifHR	−4.008	35.82	0.991	15	1.78	15.9	74,945	1.77

^aUsed to quantify two-species mixtures of G. sulfurreducens and N. commune.

^bUsed to quantify environmental samples.

^cNumber of points in calibration.