Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E22 | REPEC serotype O103:H2, Rifr | 42 |
| E22ΔgspD | E22 ΔgspD, Rifr | This study |
| E22ΔgspK | E22 gspK::Kanr, Rifr | This study |
| E22ΔsslE | E22 ΔsslE, Rifr | This study |
| E2348/69 | EPEC serotype O127:H6 | 40 |
| E2348/69ΔgspD | E2348/69 ΔgspD | This study |
| E2348/69ΔgspK | E2348/69 gspK::Kanr | This study |
| E2348/69ΔsslE | E2348/69 ΔsslE | This study |
| H10407 | ETEC serotype 078:H11, LT+, ST+ | 22 |
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR thiA | 10 |
| MT2 | H10407 gspK::Kanr | 61 |
| Plasmids | ||
| pACBSR | Medium-copy-no. vector, p15A ori, Cmr | 29 |
| pACYC184 | Medium-copy-no. vector, p15A ori, Cmr, Tcr | 12 |
| pBAD24 | Medium-copy-no. vector, ori pMB1, Apr | 26 |
| pBR322 | Medium-copy-no. vector, ori pMB1, Apr, Tcr | 8 |
| pCP20 | Low-copy-no. vector, ori R101, repA101ts, flp, Apr, Cmr | 18 |
| pFT-A | Low-copy-no. vector, ori R101 repA101ts, flp, Apr | 51 |
| pGEM-T Easy | High-copy-no. vector, ori pMB1, Apr | Promega |
| pJP15 | sslEE2348/69 in pSU39, Kanr | This study |
| pJP16 | sslEE22 in pSU39, Kanr | This study |
| pJP33 | hltA promoter and hltB from H10407 in pBR322, Apr | This study |
| pJP49 | gspKE22 in pACYC184, Cmr | This study |
| pJP51 | gspDE22 in pACYC184, Cmr | This study |
| pJP52 | gspDE2348/69 in pACYC184, Cmr | This study |
| pJP115 | micA promoter from E2348/69 in pMU6400, Tpr | This study |
| pJP168 | PBAD promoter and araC replaced by PtetA and tetR gene from Tn10 in pBAD24, Apr | This study |
| pJP169 | sslEE2348/69-tetraCys in pJP168, Apr | This study |
| pJP183 | pBR322 with the promoter and NH terminus of the Tcr gene deleted, Apr | This study |
| pKD4 | FRT-flanked Kanr gene, Kanr, Apr | 18 |
| pKD46 | Low-copy-no. vector, ori R101, repA101ts, PBAD-λ Red, Apr | 18 |
| pMU2386 | Single-copy-no. translational fusion vector, Tpr | 5 |
| pMU6400 | Synthetic RBS with ATG start codon fused in phase with codon 8 of lacZ in pMU2386, Tpr | This study |
| pSU39 | Medium-copy-no. vector, p15A ori, Kanr | 7 |
a
Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Kanr, kanamycin resistance; Rifr, rifampin resistance; Tcr, tetracycline resistance; Tpr, trimethoprim resistance.