Fig 4.

(A) Mice were infected with either WT (circles) or MGM1990 (triangles) bacteria. CFU in both lungs were determined on days 1, 8, 21, 56, and 126 after infection. The combined results of two experiments are shown. (B) In vivo loss of mmaA3-4. The percentage of MGM1990 bacteria retaining the attB-integrated mmaA3-mmaA4 cassette during mouse infection was determined by plating of bacteria on plates with and without streptomycin at the indicated time points. MGM1985 is wild-type M. tuberculosis with an empty attB-integrating streptomycin-selected cassette. (C) Mice were infected with either MGM1985 (WT; circles), MGM1995 (unsaturated alpha mycolates, no oxygenated mycolates; squares), or MGM1998 (all three mycolate classes present, no cyclopropanes; triangles). CFU in both lungs were determined on days 1, 8, 21, 56, and 126 after infection. Each time point represents the mean for 4 or 5 mice, and error bars show standard deviations (SD). (D) Bacterial loads on days 8 and 21 after infection are shown for the experiment in panel C.