Fig 5.

Gr1⁺ CD11b⁺ MDSCs harvested from day 3 (D3) septic mice produce more NO and exhibit lower arginase activity than MDSCs harvested from day 12 (D12) septic mice. Gr1⁺ CD11b⁺ MDSCs were purified from the bone marrow of a separate group of mice at day 3 and day 12 after CLP. Cells (2 × 10⁶) were cultured with 1 μg/ml LPS for 24 h. (A) Supernatants were collected and analyzed for NO levels (measured as nitrite). The cells were lysed and analyzed for arginase activity. (B) Levels of TNF-α, IL-6, IL-10, and TGF-β were also measured in the culture supernatants. Values represent the means ± SD for 7 mice per group. *, P < 0.05.