Fig 2.
Acute T. gondii infection-induced oxidative stress response and products of peroxidation. (A) Three mice of each gestation day and three placentas or blood samples from each pregnant mouse were picked for analysis by real-time PCR. Fifty milligrams of tissue or 107 nucleated cells were used for DNA extraction. Parasite burden is presented as copies per mg (placenta) or 106 cells (blood). Analyses of GSH (B), MDA (C), and 8-OHdG (D) levels were conducted per the manufacturer's instructions. The results of each gestation day are shown as mean ± SD (n = 6), and results were compared by one-way ANOVA; the SNK multiple comparison posttest was used to compare levels of various gestational days.