Fig 3.

Immunoblot demonstrating reduced EhMSP-1 protein in E. histolytica G3 strain trophozoites. (Left) Lysates from EhMSP-1-silenced and G3 trophozoites transfected with empty plasmid (G3 empty vector) were separated under nonreducing conditions by SDS-PAGE, transferred to a PVDF membrane, and probed with affinity-purified anti-EhMSP-1 rabbit polyclonal IgG and an anti-rabbit IgG–HRP conjugate secondary antibody. As a loading control, the same blot was reprobed with a rabbit polyclonal anti-ICP-1 IgG antibody. (Right) Immunoblot of G3 empty vector control E. histolytica and Entamoeba dispar strain SAW760 lysates.