Table 1.

Attenuated mutants identified by IVASS

PEST		Domain 1		Domain 2		Domain 3		Domain 4 N-terminal region
LLO mutanta	Blood plate phenotypeb	LLO mutant	Blood plate phenotype	LLO mutant	Blood plate phenotype	LLO mutant	Blood plate phenotype	LLO mutant	Blood plate phenotype
S44A	+	I61A	−	Y98A	−	I204A	−c	I421A	+
P45A	+	D62A	−	Y406A	o	Y206A	−	Y427A	o
A47G	+	I65A	−	T409A	−	Q216A	−	N432A	o
P49A	+	N119A	−			K220A	−
K50A	+	Y126A	−			F221A	−
P52A	+	L145A	−			G222A	−
K55A	−	R149A	−			F225A	−
		D157A	−			N229A	−
		K175A	−			V234A	−
		L185A	−			F236A	−
		W189A	−			G237A	−
		Y197A	−			I239A	−
		Y255A	−			V248A	−
		Y256A	−			F251A	−
		N261A	−			Q253A	−
		F269A	−			Y303A	−
		F270A	−			A318G	−
		L279A	−			F319A	−
		Y292A	−			I337A	−
		Y298A	−			I338A	−
		G299A	−			K344A	−
						G349A	o
						G350A	−
						I359A	+
						A374G	WT
						R378A	−

^aAvirulent mutants were not detected in the livers and spleens of mice 48 h postinfection.

^bQualitative hemolysis activity was tested by streaking the individual mutant strains on sheep blood agar plates and comparing zones of hemolysis with those of wild-type bacteria. The activities are reported as follows: +, hyperhemolytic; −, hypohemolytic; o, ahemolytic. WT, wild type.

^cPhenotype after extended incubation (8 to 9 days).