Table 1.
Attenuated mutants identified by IVASS
PEST |
Domain 1 |
Domain 2 |
Domain 3 |
Domain 4 N-terminal region |
|||||
---|---|---|---|---|---|---|---|---|---|
LLO mutanta | Blood plate phenotypeb | LLO mutant | Blood plate phenotype | LLO mutant | Blood plate phenotype | LLO mutant | Blood plate phenotype | LLO mutant | Blood plate phenotype |
S44A | + | I61A | − | Y98A | − | I204A | −c | I421A | + |
P45A | + | D62A | − | Y406A | o | Y206A | − | Y427A | o |
A47G | + | I65A | − | T409A | − | Q216A | − | N432A | o |
P49A | + | N119A | − | K220A | − | ||||
K50A | + | Y126A | − | F221A | − | ||||
P52A | + | L145A | − | G222A | − | ||||
K55A | − | R149A | − | F225A | − | ||||
D157A | − | N229A | − | ||||||
K175A | − | V234A | − | ||||||
L185A | − | F236A | − | ||||||
W189A | − | G237A | − | ||||||
Y197A | − | I239A | − | ||||||
Y255A | − | V248A | − | ||||||
Y256A | − | F251A | − | ||||||
N261A | − | Q253A | − | ||||||
F269A | − | Y303A | − | ||||||
F270A | − | A318G | − | ||||||
L279A | − | F319A | − | ||||||
Y292A | − | I337A | − | ||||||
Y298A | − | I338A | − | ||||||
G299A | − | K344A | − | ||||||
G349A | o | ||||||||
G350A | − | ||||||||
I359A | + | ||||||||
A374G | WT | ||||||||
R378A | − |
Avirulent mutants were not detected in the livers and spleens of mice 48 h postinfection.
Qualitative hemolysis activity was tested by streaking the individual mutant strains on sheep blood agar plates and comparing zones of hemolysis with those of wild-type bacteria. The activities are reported as follows: +, hyperhemolytic; −, hypohemolytic; o, ahemolytic. WT, wild type.
Phenotype after extended incubation (8 to 9 days).