Table 2.
Plaque sizes of LLO domain 1 mutants
Mutated residuea | Plaque sizeb |
---|---|
Y126A | 65.0 ± 5.4** |
R149A | 14.8 ± 2.8*** |
K175A | 44.1 ± 1.1*** |
L185A | 47.5 ± 1.5*** |
Y197A | 18.1 ± 3.0* |
Y255A | 66.7 ± 4.7** |
Y256A | 8.1 ± 0.3**** |
F269A | 33.7 ± 4.3** |
F270A | 47.8 ± 4.0** |
L279A | 44.3 ± 4.2** |
Y292A | 49.8 ± 5.9** |
Y298A | 21.6 ± 8.1** |
Each mutant strain was analyzed in at least three experiments.
Diameters of bacterial plaques is expressed as a percentage of the diameter of wild-type plaques and includes the standard deviation. Student's t test was used to analyze statistical significance versus wild-type bacteria.
, P < 0.05;
, P < 0.01;
, P < 0.001;
, P < 0.0001.