Table 2.

Plaque sizes of LLO domain 1 mutants

Mutated residuea	Plaque sizeb
Y126A	65.0 ± 5.4**
R149A	14.8 ± 2.8***
K175A	44.1 ± 1.1***
L185A	47.5 ± 1.5***
Y197A	18.1 ± 3.0*
Y255A	66.7 ± 4.7**
Y256A	8.1 ± 0.3****
F269A	33.7 ± 4.3**
F270A	47.8 ± 4.0**
L279A	44.3 ± 4.2**
Y292A	49.8 ± 5.9**
Y298A	21.6 ± 8.1**

^aEach mutant strain was analyzed in at least three experiments.

^bDiameters of bacterial plaques is expressed as a percentage of the diameter of wild-type plaques and includes the standard deviation. Student's t test was used to analyze statistical significance versus wild-type bacteria.

^*, P < 0.05;

^**, P < 0.01;

^***, P < 0.001;

^****, P < 0.0001.