Fig 1.
Derivation of PmpG303-311-specific CD4 T cell clone PmpG1.1. (A) The polyclonal T cell population resulting from the secondary in vitro stimulation of immune splenocytes with PmpG-125-500 was tested for specificity for the PmpG303-311 epitope. The T cells were activated with irradiated naïve splenocyte APC plus 2 μg/ml of the indicated peptides or the medium control, IFN-γ in 48-h culture supernatants quantified by ELISA. The data presented are from a single experiment done in triplicate. The error bars indicate SD. (B) Specificity of the PmpG1.1 T cell clone. The PmpG1.1 T cell clone was activated with immune irradiated splenocyte APC plus 1.5 μg/ml of the indicated proteins or 2 μg/ml of the indicated peptides. IFN-γ in 48-h culture supernatants was quantified by ELISA; aggregate data from two independent experiments are shown. (C) PmpG1.1 was stained for CD8a and CD4 and analyzed by flow cytometry. **, P < 0.005; ***, P < 0.0005; NS, not statistically significant. The error bars indicate SEM.