Fig 6.
Presentation of the PmpG303-311 epitope persists in vivo >6 months postinfection. PmpG1.1 was activated by naïve splenocyte APC or immune splenocyte APC from mice 3 weeks (21/22) and 6 months (185/186) postinfection, without or with exogenous UV-inactivated C. muridarum antigen (MoPn). (A) T cell clones were cultured in medium (control) or cocultured with naïve and immune irradiated splenocytes without exogenous antigen. The culture supernatants were collected at 48 h, and IFN-γ was quantified by ELISA; aggregate data from two independent experiments are shown. Error bars indicate SEM. (B) PmpG1.1 cells were cocultured with naïve or immune (6 months postinfection) irradiated splenocytes in the absence and presence of UV-inactivated C. muridarum (MoPn). The culture supernatants were collected at 48 h, and IFN-γ was quantified by ELISA. The data presented are from one experiment done in triplicate. ***, P < 0.0005; NS, not statistically significant. The error bars indicate SD.