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Binding affinities of RPS3 and NleH proteins for either NleH1 or NleH2a
| Protein |
Kd (μM) for prebound: |
|
|---|---|---|
| His-NleH1 | His-NleH2 | |
| GST | ND | ND |
| GST-RPS3 | 17 ± 5 | 15 ± 4 |
| GST-NleH1 | ND | 28 ± 8 |
| GST-NleH2 | 25 ± 4 | 24 ± 6 |
Dissociation constants (Kd) were estimated through curve fitting to the equation θ = ligand/(ligand +Kd), as described in Materials and Methods, and are given as means ± standard deviations (n = 4). ND, not detected.
