Table 1.
Gene cluster of Aromatoleum aromaticum strain EbN1 (GenBank accession number NC_006513.1) correlated with identified IAA-induced protein spots in Azoarcus evansiia
| Gene | Aromatoleum aromaticum EbN1 gene | Corresponding protein spot no.b | Putative annotation | Calculated size (amino acids) | Intergenic distance to next gene (bp) |
|---|---|---|---|---|---|
| iaaP | ebA2038 | Fusion of enoyl-CoA-hydratase and 3-hydroxyacyl-CoA dehydrogenase | 671 | 200 | |
| iaaQ | ebA2046 | TetR family transcriptional regulator | 302 | 247 | |
| iaaR | ebA2047 | GntR family transcriptional regulator | 229 | −1 | |
| iaaA | ebA2049 | 20, 22 | Beta-oxoacyl-CoA thiolase | 401 | 151 |
| iaaB | ebA2050 | 5, 6 | CoA ligase similar to o-benzoylsuccinyl-CoA synthase | 510 | 2 |
| iaaC | ebA2051 | 3, 4 | Hydantoin utilization protein A (HyaA), similar to 5-oxoprolinase | 707 | −1 |
| iaaD | ebD51 | C terminus identical to that of IaaC, possibly from partial gene duplication | 68 | −1 | |
| iaaE | ebA2053 | 15, 16 | Hydantoin utilization protein B (hyaB) | 564 | 2 |
| iaaF | ebA2055 | Acyl-CoA dehydrogenase similar to isovaleryl-CoA dehydrogenase | 377 | −1 | |
| iaaG | ebD52 | Methylmalonyl-CoA mutase C-terminal-domain-containing protein | 139 | −1 | |
| iaaH | ebA2060 | Methylmalonyl-CoA mutase subunit A | 537 | 13 | |
| iaaI | ebA2062 | 11, 14 | Medium FAD-binding subunit of molybdenum enzyme (xanthine dehydrogenase family) | 288 | −14 |
| iaaJ | ebA2063 | Small Fe/S-containing subunit of molybdenum enzyme | 157 | 14 | |
| iaaK | ebA2064 | 1,2 | Large molybdenum-containing subunit of molybdenum enzyme | 803 | 148 |
| iaaL | ebA2066 | 7 | CoA transferase family III | 397 | 220 |
| iaaM | ebA2070 | 12 | Branched-chain-amino-acid ABC transporter, periplasmic component, with N-terminal signal peptide sequence | 382 | 326 |
| ebA2073 | l-Sorbosone dehydrogenase | 752 |
a
The predicted functions of the proteins are indicated, along with their sizes and the distances of the respective gene to the following gene of the cluster. Negative numbers indicate overlapping genes (e.g., −1 for ATGA start/stop codon overlaps). Note that iaaQ and iaaR are divergent and that the 247-bp intergenic sequence between them should contain two promoters. FAD, flavin adenine dinucleotide.
b
For spots identified by gel electrophoresis analysis of the A. evansii proteome, see Fig. S1 in the supplemental material.