Table 1.

Influence of linker changes on PaNAGS kinetics

Linker changea	Mean apparent kinetic parameter value ± SE forb:					$100\times \frac{{\text{activity}}^{\text{20mM Arg}}}{{\text{activity}}^{\text{no Arg}}}$ (%)
	Acetyl-CoA		l-Glutamate
	V[acetyl-CoA]=∞ (U mg−1)	Kmacetyl-CoA (μM)	V[glutamate]=∞ (U mg−1)	Kmglutamate (mM)	KIglutamate (mM)
None (EQF) (WT)	80 ± 1	92 ± 9	136 ± 20	5.2 ± 1.4	72 ± 36	10.2 ± 3.6c
LEVLFQGP (PSsite)	55 ± 1	85 ± 5	83 ± 8	10.8 ± 1.6	95 ± 36	105.1 ± 0.6
LFQGP (PScore)	49 ± 2	63 ± 14	85 ± 5	2.7 ± 0.4	28 ± 4	124.5 ± 2.8
EAQGP	47 ± 1	25 ± 2	67 ± 5	4.9 ± 0.8	69 ± 13	156.3 ± 4.4
EAQAF	42 ± 1	68 ± 15	70 ± 11	2.6 ± 1.0	67 ± 37	58.0 ± 3.8
ERM	21 ± 1	25 ± 5	53 ± 4	5.5 ± 0.6	20 ± 2	114.0 ± 1.1
EQM	84 ± 2	31 ± 4	125 ± 5	4.3 ± 0.4	58 ± 7	94.0 ± 0.6
ERF	54 ± 1	35 ± 4	84 ± 3	2.7 ± 0.3	30 ± 3	5.6 ± 0.3
AQF	63 ± 1	35 ± 4	82 ± 5	7.7 ± 1.0	150 ± 30	9.4 ± 0.5

^aChanges as defined in the legend of Fig. 2C.

^bSee Materials and Methods for details.

^c[Arg] equals 10 mM.