Fig 2.

DipA and RbdA are essential for the dispersion response of P. aeruginosa PA14. (A) ΔdipA and ΔrbdA biofilms do not disperse in response to addition of glutamate, ammonium chloride, nitric oxide, and mercury chloride compared to wild-type P. aeruginosa PA14. Biofilms were grown in tube reactors. (B) Confocal images of ΔbifA and ΔdipA biofilms prior to and 30 min following addition of glutamate. Biofilms were grown in flow cells for 5 days before induction of dispersion. Biofilms were stained with the LIVE/DEAD BacLight viability stain (Invitrogen Corp.). White size bars = 100 μm.