Fig 7.

The EAL motifs are essential for dispersion, while the GAF domain is not essential for dispersion and biofilm formation. (A) Both EAL motifs are essential for dispersion. (B) Dispersion assays demonstrating that complementation of the ΔdipA mutant with dipA-NoGAF but not GAFonly restored the biofilm-deficient phenotype of the ΔdipA mutant to wild-type levels. (C) Biofilm architecture of biofilms expressing dipA-NoGAF. Biofilms were grown for 6 days in diluted LB medium, after which time, confocal images were acquired. Biofilms were stained with the LIVE/DEAD BacLight viability stain (Invitrogen Corp.). White size bars = 100 μm. All experiments were carried out at least in triplicate. Error bars indicate standard deviations.