Table 1.

Quantitative COMSAT analysis of biofilm architecture prior to and following induction of nutrient-induced dispersion^a

Condition and strain	Total biomass (μm3/μm2)	Thickness (μm)		Roughness coefficient
		Avg	Maximum
Prior to dispersion
PA14	34.5 ± 3.4	39.2 ± 7.8	81.3 ± 15.0	0.8 ± 0.1
ΔdipA mutant	45.1 ± 2.2	46.9 ± 2.3	95.0 ± 5.0	1.1 ± 0.1
ΔbifA mutant	51.9 ± 12.9	59.9 ± 12.2	142.1 ± 12.9	0.57 ± 0.12
Following induction of nutrient-induced dispersion
PA14	1.2 ± 0.4*	1.5 ± 0.6*	12.7 ± 3.3*	1.8 ± 0.1*
ΔdipA mutant	44.7 ± 0.9	46.9 ± 1.3	95.0 ± 5.0	1.1 ± 0.1
ΔbifA mutant	2.94 ± 0.05*	2.24 ± 0.08*	24.6 ± 8.6*	1.7 ± 0.37*

^aCOMSTAT analysis was carried out from biofilms grown in triplicate using at least 6 images per replicate. Dispersion was induced by addition of 18 mM glutamate to the growth medium. *, significantly different from biofilms prior to dispersion (P < 0.05), as determined by ANOVA and SigmaStat.