Table 2.
COMSTAT analysis of P. aeruginosa wild-type and mutant biofilms inactivated in or differentially expressing dipA and truncated dipA constructsa
| Strain name or genotype | Total biomass (μm3/μm2) | Thickness (μm) |
|
|---|---|---|---|
| Avg | Maximum | ||
| PA14 strains | |||
| PA14 | 30.10 ± 9.5 | 34.34 ± 11.5 | 104.83 ± 15.5 |
| ΔdipA mutant | 55.70 ± 11.6* | 63.30 ± 16.2* | 119.56 ± 13.8 |
| ΔdipA/pMJT-dipA | 39.76 ± 8.9 | 49.80 ± 8.9 | 103.26 ± 23.6 |
| PA14/pMJT-dipA | 12.00 ± 8.3* | 18.64 ± 15.1* | 33.14 ± 11.0* |
| PA14/pMJT-dipA-NoGAF | 27.87 ± 18.9 | 34.50 ± 21.4 | 82.0 ± 15.12* |
| ΔdipA/pMJT-dipA-NoGAF | 34.91 ± 12.8 | 46.34 ± 20.6 | 107.0 ± 23.7 |
| PA14/pMJT-GAFonly | 31.8 ± 4.4 | 40.48 ± 7.9 | 112.6 ± 38.1 |
| ΔdipA/pMJT-GAFonly | 47.2 ± 5.3* | 54.4 ± 19.5 | 102.4 ± 11.6 |
| PAO1 strains | |||
| PAO1/pMJT1 | 26.92 ± 5.6 | 37.18 ± 5.15 | 95.33 ± 19.5 |
| PAO1/pMJT-dipA | 16.0 ± 12.3* | 18.64 ± 15.1* | 53.14 ± 11.0* |
| PAO1/pMJT-dipA-NoGAF | 24.45 ± 14.4 | 26.34 ± 15.5 | 74.91 ± 13.2 |
a
COMSTAT analysis was carried out from biofilms grown in triplicate using at least 6 images per replicate. *, significantly different from the 144-h-old PA14 or PAO1 parental strain (P < 0.05), as determined by ANOVA and SigmaStat.