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Exoprotein analysis. Culture supernatants were collected at the indicated time points, and exoproteins were isolated and separated via SDS-PAGE. Cells contained the indicated agr allele and the blaZpc-blaZ construct (pEG832). (A, C) Coomassie staining. Bands indicated by arrows were analyzed by LC-MS. (B, D) Results of β-lactamase zymogram analysis performed after renaturing SDS-PAGE with the chromogenic substrate nitrocefin.
