Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or characteristic(s) | Source or reference(s) |
|---|---|---|
| S. aureus strains | ||
| UAMS-1 | Methicillin-susceptible osteomyelitis isolate (USA200) | 12, 28 |
| LAC | Community-associated methicillin-resistant isolate (USA300) | 43 |
| RN4220 | Restriction-deficient derivative of RN450 | 18 |
| U151 | UAMS-1 containing pCN51 | This study |
| EWM14 | UAMS-1 ΔSSR42 | This study |
| EWM15 | UAMS-1 ΔSSR42 containing pCN51 | This study |
| EWM16 | UAMS-1 ΔSSR42 containing pCN51::SSR42 | This study |
| EWM22 | UAMS-1 containing pEWM21 | This study |
| EWM24 | EWM14 containing pEWM21 | This study |
| EWM25 | UAMS-1 containing pEWM22 | This study |
| EWM28 | EWM14 containing pEWM22 | This study |
| JMM1 | LAC ΔSSR42 | This study |
| JMM2 | LAC ΔSSR42 containing pJMM1 (SSR42+) | This study |
| JMM3 | LAC ΔSSR42 containing pJMM2 (SSR42.mut+) | This study |
| JMM4 | LAC ΔSSR42 containing pJMM4 (SSR42.1+) | This study |
| JMM5 | LAC ΔSSR42 containing pJMM5 (SSR42.2+) | This study |
| KLA16 | UAMS-1 Δspa | 34 |
| VJT26.90 | LAC containing pXen-Phla-lux | This study |
| VJT26.91 | LAC containing pXen-Ppvl-lux | This study |
| VJT26.92 | LAC containing pXen-PlogCB-lux | This study |
| E. coli strains | ||
| DH5α | Invitrogen | |
| INVα | Invitrogen | |
| Plasmids | ||
| pCN51 | Shuttle vector | 16 |
| pCN38 | Shuttle vector | 16 |
| pEWM20 | pCN51 containing SSR42 | This study |
| pCRII | Shuttle vector | Invitrogen |
| pJMM1 | pCN38 containing SSR42 | This study |
| pJMM2 | pCN38 containing SSR42 with T-to-A transversion | This study |
| pJMM3 | pCN51 containing SSR42 with T-to-A transversion | This study |
| pJMM4 | pCN38 containing nt 1–445 of SSR42 | This study |
| pJMM5 | pCN38 containing nt 446–891 of SSR42 | This study |
| pKOR1 | Allelic replacement vector | 5 |
| pXen-1 | Luciferase reporter plasmid | Xenogen |
| pXen-Phla-lux | hla promoter fused to lux operon | This study |
| pXen-Ppvl-lux | pvl promoter fused to lux operon | This study |
| pXen-PhlgCB-lux | hlgCB promoter fused to lux operon | This study |