Table 2.
Primers used in this study
| Primer | Nucleotide sequence (5′ → 3′)a | Targetb |
|---|---|---|
| EWM-01 | GGATCCCTATTTTTTAATAAAACCTCAGCACATTATG | spa promoter (F) |
| EWM-05 | GCTAGCCATATGACCTAGTTTACGAATTGAATAAATGTTTTTCTTTTTC | spa promoter (R) |
| EWM-03 | CCGCGGTTGCAACACTCTATTATCATTTTAT | SSR42 complement (F) |
| EWM-04 | CCGCGGGGTAATAGTGAAAAATAAAAGAAAT | SSR42 complement (R) |
| EWM-52 | GCTTTATAAGCAAGGGGTGGTCAAC | pCN51 (F) |
| EWM-53 | GAAAATAAATCTCGAAAATAATAGAGGG | pCN51 (R) |
| EWM-11 | GGGGACCACTTTGTACAAGAAAGCTGGGTTTGCAACACTCTATTATCATTTTAT | SSR42 upstream (F) |
| EWM-111 | GTTTCACCGCGGATCTATCTCTTTCTTTTTGTGTTTA | SSR42 upstream (R) |
| EWM-112 | TAAACGCCGCGGGCTTTATTTTTTAGTAGATTGTTTA | SSR42 downstream (F) |
| EWM-113 | GGGGACAAGTTTGTACAAAAAAGCAGGCTGGTAATAGTGAAAAATAAAAGAAAT | SSR42 downstream (R) |
| EWM-147 | GGTACCTTGCAACACTCTATTATCATTTTAT | SSR42 (F) |
| EWM-148 | GAATTCGGTAATAGTGAAAAATAAAAGAAAT | SSR42 (R) |
| EWM-179 | CCTCAGTTTATGGCGCAAGAGGTTC | cap5A (F) |
| EWM-180 | GTGCGACTTTAACTGCTGTACCGTC | cap5A (R) |
| EWM-221 | GCAGAAGCTAAAAAGCTAAATGATG | spa (F) |
| EWM-222 | GCTCACTGAAGGATCGTCTTTAAGG | spa (R) |
| 16S-L | TAACCTACCTATAAGACTGGGATAA | 16S (F) |
| 16S-R | GCTTTCACATCAGACTTAAAA | 16S (R) |
| JMM-1 | GTGCATTGGACAACGAAATTCACCATCAA | SSR42 (F) |
| JMM-2 | CGCATCTCTTGATGTAAAATCTAAGATGTTTATG | SSR42 (R) |
| JMM-3 | GGGGAGTAATTTTAAGTAATATCTTGTTGCTGCT | SSR42 (R) |
| JMM-4 | GCTAACACCTAACATCAAAGAATATTCATCAA | SSR42 (F) |
| JMM-5 | GGGAGTAATTTTAAGTAATATCTTGTTGCT | SSR42 (R) |
| JMM-6 | GAATTCGGTAATAGTGAAAAATAAAAGAAAT | SSR42 (F) |
| JMM-7 | GTTTCACCGCGGATGCCTGTCTTGATGAAGTTATCTATGAT | SSR42 (R) |
| JMM-8 | TAAACGCCGCGGTTTGTGTTTAAAACACGACTTTGTAGT | SSR42 (F) |
| JMM-9 | GGTACCATTAACATTAATTATCGGAGGTAATTTTATG | SSR42 (R) |
| JMM-10 | GTTTCACCGCGGCTTTATTTTTTAGTAGATTGTTTAAAATTTCTTAGT | SSR42 (R) |
| JMM-11 | TAAACGCCGCGGCCACATCTAAGAAACTCATCTAGAA | SSR42 (F) |
| JMM-12 | GAGCACTTTATCACCAGCAGCATTAG | aur (F) |
| JMM-13 | CGGATTTAGGCAATTCTTTTAATGCTTTGA | aur (R) |
| JMM-14 | GGGCACACTGATTGGAGTAACAGTT | isaB (F) |
| JMM-15 | CCAGAATAAAATTAGCATTATTACCAATATAACCATT | isaB (R) |
| JMM-16 | GGTTATTGGTGGTGCATTCACGGTA | sraP (F) |
| JMM-17 | CCGAACTACTATTTTTCGTTACACTTGTGGAAT | sraP (R) |
| JMM-18 | CACCTGTAAGTGAGAAAAAGGTTGATGAT | lukF-PV (F) |
| JMM-19 | GCCAGAATAAATGTTTCCAGCAGCTTT | lukF-PV (R) |
| JMM-22 | CCGGTACTACAGATATTGGAAGCAATA | hla (F) |
| JMM-23 | CCAGCAATGGTACCTTTCGTTCTAATAA | hla (R) |
| JMM-24 | GGGGCGTGACTCAAAATATTCAATTTGA | hlgC (F) |
| JMM-25 | GGCCATCGCATAGCTTTAACATGATTA | hlgC (R) |
a
Nucleotides in italics indicate attB sites, and underlined nucleotides represent indicated restriction sites.
b
F, forward; R, reverse.