Fig 6.
Effect of HetR on DNase I footprinting of NtcA on DNA sequences between the two TSPs of the devB gene. DNase I footprinting assays were carried out with purified NtcA (1 μM) and HetR, as indicated, and a DNA fragment of the devB promoter region that was amplified by PCR using primers alr3710-17 (unlabeled) and alr3710-19 (32P labeled) (lower strand) in the presence of 0.6 mM 2-OG. Except for NtcA and HetR, final concentrations of all components were the same in the different reaction mixtures. Vertical lines denote windows of protection. Arrowheads point to some hypersensitive positions.