Table 1.
Sequences of primers used in this study
| Target and primer name | Sequencea |
|---|---|
| escA deletion mutant | |
| ESCA-01F | CGGGTACCAAAAGCGCGTATTGGTGC |
| ESCA-01R | CGGCTAGCTAAAATTCTGTCCAACATATTCA |
| ESCA-02F | TTTGCTAGCAAGGAACATTACTTTGACTAGCA |
| ESCA-02R | CAGAGCTCTACGGTTGCTAAAGGGTTATTAA |
| pEscA (pCR2.1-TOPO) | |
| EscA-F1 | AGAAGCTTCGAAAAAACGATTGAAAGCC |
| EscA-2HA-R | AACTCGAGGTCAAAGTAATGTTCCTTTATGG |
| Δ19EscA (pCR2.1-TOPO) | |
| Δ19EscA-F | AAAGCTTAGCGAACCGATTGAGAG |
| pEscA (pACYC184) | |
| EscA-F2 | CTGGATCCAACAAAGCACCAAAGATATC |
| EscA-HSV-R1 | AGAAGCTAGCGTCAAAGTAATGTTCCTTTATGG |
| EscA-HSV-R2 | GGTCGACTCAATCCTCGGG |
| pEscC (pCR2.1-TOPO) | |
| pEscC-F | CCAAGCTTGTTTTCTGATATAGGACG |
| pEscC-2HA-R | GGCTCGAGTTCGCTAGATGCAG |
| Δ21PhoA-2HA (pMAC2) | |
| Δ21PhoA-F | GTACGGTACCATTAAAGAGGAGAAATTAACTGTGCGGACACCAGAAATGCC |
| PhoA-R | TTAAGCGTAATCTGGAACATCGTATGGGTATTTCAGCCCCAGAGCGGC |
| HA-R | TAGAGCTCTTAAGCGTAATCTGGAACATCGTA |
| EscA19PhoA-2HA (pMAC2) | |
| EscA19PhoA-F1 | GTTGGACAGAATTTTATCTATTCGTAAAAGCAGAGCGAACCGATTGAGAGAATCACGGACACCAGAAATGCCTGT |
| EscA19PhoA-F2 | GTACGGTACCATTAAAGAGGAGAAATTAACTATGTTGGACAGAATTTTATCTATTCG |
a
Restriction sites are underlined.