Fig. 3.

Impact of siRNAs on viral DNA replication and virus spreading. (A) A549 cells were transfected with siRNAs directed against the E1A, DNA polymerase (Pol), pTP, IVa2, hexon (Hex), and protease (Prot) genes, or a non-targeting control siRNA (neg. ctrl.) at a concentration of 10 nM, and then infected with Ad5 at an MOI of 0.01 TCID₅₀/cell. Virus genome copy numbers were determined at 48 h post-infection by qPCR, using E1A-specific primers. Values represent mean ± SD of three independent experiments, each performed in triplicate. For each experiment, real-time qPCR quantification was performed in duplicate. ^*p < 0.001. (B) Long-term infection of A549 cells with Ad5 at an MOI of 0.01 TCID₅₀/cell, and treatment with 10 nM of the indicated siRNAs. Virus genome copy numbers were determined at time points 0, 2, 4, and 6 days post-infection by qPCR. Representative data from at least three independent experiments, each performed in triplicates, are shown (mean ± SD; n = 3). For each experiment, real-time qPCR quantification was performed in duplicate. ^**p < 0.01.