Fig. 4.

siRNAs decrease the numbers of infectious virus particles. A549 cells were transfected with siRNAs directed against the E1A, DNA polymerase (Pol), pTP, IVa2, hexon (Hex), and protease (Prot) genes, or a non-targeting control siRNA (neg. ctrl.) at a concentration of 10 nM, and then infected with Ad5 at an MOI of 0.01 TCID₅₀/cell. Numbers of infectious virus particles at 48 h post-infection were determined on A549 cells by TCID₅₀ assays. Representative data from three independent experiments, each performed in triplicate, are shown (mean ± SD; n = 3). ^**p < 0.01, ^***p < 0.001.