Supplementary Figure 2.

Comparison of the activity two siRNAs that performed best in terms of inhibition of virus replication, i.e., Pol-si2 (A) or protecting infected cells from cell death, i.e., E1A-si3 (B) to that of their respective scrambled controls. A549 cells were transfected with siRNAs Pol-si2 (Pol), E1A-si3 (E1A), a universal non-targeting negative control siRNA (neg. ctrl.), or scrambled versions of the two targeting siRNAs (scrambled Pol, scrambled E1A) at a concentration of 10 nM. Control cells receiving only Lipofectamine 2000 (Lipo) or fresh medium instead of any components of the transfection mix (mock) were included as well. Transfected cells were infected with Ad5 at an MOI of 0.01 TCID₅₀/cell and virus genome copy numbers were determined at 48 h post-infection by qPCR, using E1A-specific primers. Values represent mean ± SD of two independent experiments, each performed in triplicate. For each sample, real-time qPCR quantification was performed in duplicate. ^***p < 0.001. Not significant (ns).