Supplementary Figure 6.

SiRNAs do not alter the viability of uninfected cells. A549 cells were transfected with siRNAs directed against the E1A, DNA polymerase (Pol), pTP, IVa2, hexon (Hex), and protease (Prot) genes, a universal non-targeting negative control siRNA (neg. ctrl.), and scrambled versions of the E1A and DNA polymerase siRNAs (scrambled E1A, scrambled Pol) at a concentration of 10 nM. At 6 days post-transfection, cells were subjected to MTS assay analysis. The viability of transfected cells was calculated in relation to the viability of cells treated only with Lipofectamine 2000. A representative example (mean ± SD; n = 3) of one out of several control experiments performed in triplicates, each, is shown.