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. 2012 Apr 20;19(4):498–506. doi: 10.1016/j.chembiol.2012.03.006

Figure 3.

Figure 3

Essentiality of MSMEG0250 in M. smegmatis mc2155

Growth of the conditional mutant ΔMSMEG0250 and parental merodiploid strain (mc2155::pMV306mmpL3) on Tryptic Soy Agar with or without the inducer acetamide. Ten microliters of 10-fold serial dilutions of cultures was spotted on the agar plates and incubated for 5 days at 37°C.