Figure 4.

Lipid Analysis of the ΔMSMEG0250 Conditional Mutant

(A) 2D-TLC analysis of [¹⁴C]-labeled lipids from the ΔMSMEG0250 conditional mutant. Cultures were grown and labeled in tryptic soy broth in the presence or absence of acetamide. Petroleum ether extracts (Fraction-I) and intracellular apolar lipids (Fraction-II) were separated on using chloroform:methanol:water (100:14:0.8) in Direction 1 and chloroform:acetone:methanol:water (50:60:2.5:3) in Direction 2. Positions of TMM and TDM are indicated by dotted and solid arrows, respectively.

(B) TLC analysis of methyl esters of [¹⁴C]-labeled, cell wall bound mycolic acids (MAMES) separated using petroleum ether:acetone (95:5) as the solvent system. Methyl esters of the different subclasses of mycolic acids are indicated.

See also Figure S1.